Given the high number of GMOs currently authorised or whose authorisation is pending or has expired, it is necessary to implement an optimised strategy for screening food and feed samples in the EU market. The JRC GMO-Matrix web application, available on the website of the European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF), may help designing such strategies. This web application provides in silico e-PCR predictions of the GM events possibly detected by the EU reference methods in the GMOMETHODS database. By using the JRC GMO-Matrix application, the EURL GMFF proposes in this report, screening strategies for detecting all EU authorised GMOs in samples containing cotton, oilseed rape, maize, soybean or sugar beet. These proposals may contribute to the design of more efficient approaches for EU legislative enforcement and inspection control.